Data Sheet

Name：CHOLERA TOXIN B SUBUNIT,

Catalog:abs80001

CAS No:131096-89-4

Synonyms: Choleragenoid; CTB；CTxB，

Form：lyophilized powder

Size： 1mg

Purity：≥95% (SDS-PAGE)

Storage Temperature：2-8°C (Store the lyophilized powder and reconstituted solutions at 2–8 °C) Solubility：H2O: soluble 1mg/mL (stable indefiniy at 2-8 °C)

Reconstitution： Reconstitute with water to a final concentrationof 1 mg cholera toxin B subunit per ml. Swirl bottles

gently during reconstitution. Avoid vigorous pipetting of solutions that may lead to foaming.Solutions can be filtered through a 0.2 mm filter.

Description

Cholera toxin belongs to the AB5 –subunit family oftoxins.The native hexameric protein has a molecular mass of ~85 kDa and contains two subunits. It consists of a single A subunit (~27.2 kDa), responsible for the ADP-ribosylation activity, and five B subunits(~11.6 kDa each), which are arranged as a pentameric ring with an apparent 5-fold symmetry and are associated with the cell surface receptor binding and subsequent internalization (transmembrane transport)of the enzymatic component.

Application

Cholera toxin and its purified A subunit are frequently used for the study of signal transduction mechanisms. In addition, cholera toxin acts as an adjuvant through the stimulation of B lymphocytes.

The cholera toxin B subunit alone is used for track tracing in neurological research, taking advantage of GM1 ganglioside binding and retrograde transport.Tissue culture cells treated with cholera toxin are not killed and tissues of animals do not become necrotic.

The B subunit is non-toxic to cells and possesses no intrinsic adenylate cyclase activity. The cholera toxin B subunit (CTB) attaches to cells by binding to ganglioside GM1.8 CTB has been shown to be a good label for microglial cells

(due to the enrichment of ganglioside GM1 on their cell surface), but not for oligodendrocytes or astrocytes. The B subunit has been reported to be an excellent tracer for the study of axonal transport using immunohistochemical methods. Recently it has been widely used as a marker of membrane lipid rafts, which are membrane microdomains enriched with cholestrol and sphingolipids. These lipid rafts have an important role in cell signaling and protein

trafficking.

Storage/Stability

The product, as supplied, is stable 2 years when stored properly.

Solutions are stable for at least 3 months when stored at 2–8 °C. DO NOT FREEZE.

References

1.  Nedelkoska, L., and Benjamins, J.A., Binding of cholera toxin B subunit: a surface marker for

murine microglia but not oligodendrocytes or astrocytes. J. Neurosci. Res., 53, 605-612 (1998).

2.  Janes, P.W., et al., Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor. J. Cell Biol., 147, 447-461 (1999).

Research Use only  

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